Oznaczanie wybranych mikotoksyn w żywności. Cz. II. Dobór optymalnych warunków oznaczania fumonizyn B1 i B2 w przetworach z kukurydzy metodą wysokosprawnej chromatografii cieczowej
[Determination of selected mycotoxins in food. Part II. Selection of optimised conditions for the determination of fumonisin Bi and B2 in corn products by means of high-performance liquid chromatography]

ABSTRACT

The aim of this study was to perform a optimised method for determination of fumonisins B₁ and B₂ in corn products. The manner of extraction and clean-up of com products extracts as well conditions of reaction of fumonisins with OPA was described. The main steps of optimised analytical procedure were: extraction of sample with methanol and water (3+1), clean-up of extracts on SAX column, derivatisation with OPA, and determination by means of RP-HPLC. The mobile phase was a mixture of methanol, water and acetic acid (75+24+1). Fluorometric detection was made at 370/440 nm. The mean recovery of fumonisins dependent on fortification level and product was 64-95%, limit of detection for each of fumonisins was 15 µg/kg.