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Rocz Panstw Zakl Hig 2005, 56(3): 267-273

Badanie aktywności lipolitycznej Thermomyces lanuginosus na tributyrynie
[A study of the Thermomyces lanuginosus lipolytic activity on tributyrin]

K. Janda, A. Stolarska

STRESZCZENIE
Celem pracy było porównanie aktywności lipolitycznej szczepów termofilnego grzyba Thermomyces lanuginosus, wyizolowanych z surowego ziarna kawy, łuskanych orzechów laskowych, podłoża pieczarkowego, biohumusu, kompostu ogrodowego i kompostu liściowego. Aktywność lipolityczną szczepów grzybowych testowano przy użyciu tributyryny. Wszystkie badane szczepy zdolne były do hydrolizy tego substratu. Najwyższą aktywnością lipolityczną charakteryzowały się szczepy wyizolowane z surowego ziarna kawy.

 

ABSTRACT
The study was to compare the lipolytic activity of Thermomyces lanuginosus wild strains on the medium containing 1-% tributyrin. The strains were isolated from raw coffee beans, shelled hazelnuts, mushroom compost, biohumus, garden compost and leaf compost. The incubation was carried out at 55oC for ten days. The lipolytic activity index was the hydrolysis zone diameter/colony diameter ratio. Daily growth rates and daily clearance zone increase were also analyzed. All strains tested hydrolyzed tributyrin. The highest lipolytic activity index values were found in the strains isolated from raw coffee beans. Relatively high lipolytic activity index values were also found in the strains isolated from shelled hazelnuts. The highest lipolytic activity indices were observed between the 3rd and 5th day of incubation. It was hypothesized that the products of the tributyrin hydrolysis considerably affected both fungal growth and lipolytic activity. The T. lanuginosus strains isolated from raw coffee beans and shelled hazelnuts have a great biotechnological potential.

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