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Rocz Panstw Zakl Hig 1999, 50(4): 409-419

Oznaczanie witamin B1 i B2 w wybranych produktach owocowo-warzywnych
[Determination of vitamins B1 and B2 in selected vegetable-fruit products]

L. Czerwiecki , G. Wilczyńska

ABSTRACT

A simple method was described to determine vitamins Bi and B2 in fruit and vegetable-fruit juices. Vitamins were extracted with perchloric acid and for their determination an ion pairing RP-HPLC technique with UV detection by 254 nm was applied. The average recovery for vitamin Bi was 85-104% and 94-105% for vitamin B2. The statitically estimated limits of identification and detection were respectively, for both vitamins: for Bi: 0,008 and 0,0024, and for B2 0,0002 and 0,0007 mg/ml of product.

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